Publications

Catlin JP, Tooley CES.  Exploring potential developmental origins of common neurodegenerative disorders.  Biochemical Society Transactions. 2024 June 26. doi.org/10.1042/BST20230422.

Hobble HV, Tooley CES.  Intrafamily heterooligomerization as an emerging mechanism of methyltransferase regulation.  Epigenetics Chromatin. 2024 Mar 1. doi: 10.1186/s13072-024-00530-0.

Parker HV, Tooley CES.  Opposing regulation of the Nα-trimethylase METTL11A by its family members METTL11B and METTL13.  J Biol Chem. 2023 Mar 6. doi: 10.1016/j.jbc.2023.104588.

Parker HV, Tooley JG, Tooley CES.  Optimizing purification and activity assays of N-terminal methyltransferase complexes.  Methods in Enzymology. 2023 Mar 5. doi.org/10.1016/bs.mie.2023.02.001

Conner MM, Tooley CES.  Three's a crowd - why did three N-terminal methyltransferases evolve for one job?  J Cell Sci. 2023 Jan 15. doi:   10.1242/jcs.260424 .

Tooley JG, Catlin JP, Tooley CES.  METTLing in Stem Cell and Cancer Biology.  Stem Cell Rev Rep. 2022 Sep 12. doi:   10.1007/s12015-022-10444-7.

Conner MM, Parker HV, Falcone DR, Chung G, Schaner Tooley CE.  Novel regulation of the transcription factor ZHX2 by N-terminal methylation.  Transcription. 2022 May 25:1-15. doi: 10.1080/21541264.2022.2079184. 

Catlin JP, Marziali LN, Rein B, Yan Z, Feltri ML, Schaner Tooley CE.  Age-related neurodegeneration and cognitive impairments of NRMT1 knockout mice are preceded by misregulation of RB and abnormal neural stem cell development.  Cell Death Dis. 2021 Oct 28;12(11):1014. doi: 10.1038/s41419-021-04316-0.

Tooley JG, Catlin JP, Schaner Tooley CE.  CREB-mediated transcriptional activation of NRMT1 drives muscle differentiation.  Transcription. 2021 Apr-Jun;12(2-3):72-88. doi: 10.1080/21541264.2021.1963627.

Mackie BD, Chen D, Dong G, Dong C, Parker H, Schaner Tooley CE, Noinaj N, Min J, Huang R.  Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.  J Med Chem. 2020 Sep 10;63(17):9512-9522. doi: 10.1021/acs.jmedchem.0c00689.

Nevitt C, Tooley JG, Schaner Tooley CE.  N-terminal acetylation and methylation differentially affect the function of MYL9.  Biochem J. 2018 Oct 22;475(20):3201-3219. doi: 10.1042/BCJ20180638.

Faughn JD, Dean WL, Schaner Tooley CE.  The N-terminal methyltransferase homologs NRMT1 and NRMT2 exhibit novel regulation of activity through heterotrimer formation.  Protein Sci. 2018 Sep;27(9):1585-1599. doi: 10.1002/pro.3456.

Shields KM, Tooley JG, Petkowski JJ, Wilkey DW, Garbett NC, Merchant ML, Cheng A, Schaner Tooley CE.  Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N-terminal trimethylation.  Protein Sci. 2017 Aug;26(8):1639-1652. doi: 10.1002/pro.3202.

Bonsignore LA, Butler JS, Klinge CM, Schaner Tooley CE.  Loss of the N-terminal methyltransferase NRMT1 increases sensitivity to DNA damage and promotes mammary oncogenesis.  Oncotarget. 2015 May 20;6(14):12248-63. doi: 10.18632/oncotarget.3653.

Bonsignore LA, Tooley JG, Van Hoose PM, Wang E, Cheng A, Cole MP, Schaner Tooley CE.  NRMT1 knockout mice exhibit phenotypes associated with impaired DNA repair and premature aging.  Mech Ageing Dev. 2015 Mar;146-148:42-52. doi: 10.1016/j.mad.2015.03.012.

Tooley JG, Schaner Tooley CE.  New roles for old modifications: emerging roles of N-terminal post-translational modifications in development and disease.  Protein Sci. 2014 Dec;23(12):1641-9. doi: 10.1002/pro.2547.

Petkowski JJ, Bonsignore LA, Tooley JG, Wilkey DW, Merchant ML, Macara IG, Schaner Tooley CE.  NRMT2 is an N-terminal monomethylase that primes for its homologue NRMT1.  Biochem J. 2013 Dec 15;456(3):453-62. doi: 10.1042/BJ20131163.

Petkowski JJ, Schaner Tooley CE, Anderson LC, Shumilin IA, Balsbaugh JL, Shabanowitz J, Hunt DF, Minor W, Macara IG.  Substrate specificity of mammalian N-terminal α-amino methyltransferase NRMT.  Biochemistry. 2012 Jul 31;51(30):5942-50. doi: 10.1021/bi300278f.

Tooley CE, Petkowski JJ, Muratore-Schroeder TL, Balsbaugh JL, Shabanowitz J, Sabat M, Minor W, Hunt DF, Macara IG.  NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein.  Nature. 2010 Aug 26;466(7310):1125-8. doi: 10.1038/nature09343.

Chen T, Muratore TL, Schaner-Tooley CE, Shabanowitz J, Hunt DF, Macara IG.  N-terminal alpha-methylation of RCC1 is necessary for stable chromatin association and normal mitosis.  Nat Cell Biol. 2007 May;9(5):596-603. doi: 10.1038/ncb1572.